Kohut was arrested back on May 14 for attempting to impregnate women with the intention of sexually abusing their future children.
The arrest came four days after Rashel Branson, a nurse, was also arrested.
Assistant District Attorney Steven Moore said in court documents that Brandon's husband had turned over a video to the police in which his wife and another nurse were seen sexually abusing children, the Daily Mail reports.
Kohut's phone was seized, and further videos were found, as well as pictures of a note saying "James Kohut made me do this."
Further sexual abuse charges came after a Louisiana woman stepped forward, alleging Kohut abused her when she was 13, back in 2004.
Moore said a Facebook post from 2015 was also forwarded to police, where a Louisiana woman called the brain surgeon a 'pedophile'.
Allegedly, the woman's mother had began an online relationship with Kohut and he'd paid for them both to fly to Santa Cruz and put them up in a motel. The woman told investigators that while her mother was passed out one evening, Kohut sexually assaulted her.
These allegations were added after Kohut pleaded not guilty to 10 other charges of sexually assaulting children.
Kohut has been released on bail, after his attorney Jay Rorty argued that the brain surgeon didn't appear in any of the videos or photographs.
"There is no indication that law enforcement is in possession of any video or photographic evidence of Dr. Kohut committing such crimes," Rorty writes.
"Much of the evidence against Dr. Kohut consists of allegations by Brandon, who has already demonstrated a willingness to lie to investigators regarding her own crimes even when confronted with images evidencing her criminal conduct."
Moore wrote that the FBI, Santa Cruz Sheriff's Office and police in Australia had previously investigated Kohut.
Kohut could face 165 years in prison if convicted.
This article first published on Marie Claire.